Screening of Endophytic Bacteria from Purwoceng Roots (Pimpinella pruatjan Molk.) Antibacterial Activity Against Staphylococcus aureus

Sartika GP(1*),

(1) Universitas Sembilanbelas November Kolaka
(*) Corresponding Author

Abstract


Most antimicrobial agents, antifungus or antibacterial have been isolated from plants. Purwoceng (Pimpinella pruatjan Molk.) is one of the potential plants that produced an antibacterial agent. But, the status of purwoceng as an endemic plaint in Central Java is "endangered". The endophytic bacteria of the roots can produce an antibacterial agent. The research was performed by purified the endophytic bacteria isolate, the antagonism test as an antibacterial against Staphylococcus aureus also performed, and the data obtained were analyzed descriptively

 

Keywords:  Endophytic Bacteria,  Pimpinella pruatjan Molk., Staphylococcus aureus


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References


Boucher, H.W. and Corey, G.R.. (2008). Epidimiology of Methicillin-Resistant Staphylococcus aureus. CID. Vol.46(5): 344-349.

Brooks, G.F., J.S. Butel, S.A. Morse. (2004). Medical Microbiology 23rd edition. McGraw-Hill Companies Inc, New York.

Caropeboka, A.M. dan I. Lubis. (1975). Pemeriksaan pendahuluan kandungan kimia akar Pimpinella alpine (purwoceng). Dalam Simposium Tanaman Obat I, 8- 9 Desember, Bagian Farmakologi. FKH, Institut Pertanian Bogor.

Crawford, D. L., Lynch J. M, Whipps J. M, and Ousley M.A (1993). Isolation and Characterization of Actinomycete Antagonists of a Fungal Root Pathogen. Appl Environ Microbiol. Nov; 59(11): 3899–3905.

Darwati, I., Roostika, I. (2006). Status Penelitian Purwoceng (Pimpinella alpina Molk.) di Indonesia. Buletin Plasma Nutfah Vol.12 No.1.: 9.

Elita, A., Saryono, S., Christine, J. (2013). Penentuan Waktu Optimum Produksi Antimikroba dan Uji Fitokimia Ekstrak Kasar Fermentasi Bakteri Endofit Pseudomonas sp. Dari Umbi Tanaman Dahlia (Dahlia variabilis). J.Ind.Che.Acta Vol.3 (2) : 56.

Hernani dan O. Rostiana. 2004. Analisis kimia akar purwoceng (Pimpinella pruatjan). Makalah disampaikan pada Seminar Indonesian Biopharmaca and Excibition Conference. Yogyakarta, 14-15 Juli.

Infection Control and Team St. James’s Hospital. Patient/ Visitor Information Leaflet: MRSA (Meticillin-Resistant Staphylococcus aureus). St. James’s Hospital. Dublin.

Iniguez, A.L. 2004. Biology of plant-bacterial endophyte interactions. Proquest Information and Learning Company, United States 17 : 13.

Jawetz, Melnick, & Adelberg's. 2007. Medical Microbiology, 25 th edition. The McGraw-Hill Companies. New York.

Klein, E., Smith, D.L. and Laxminarayan, R. 2007. Hospitalizations and Deaths Caused by Meticillin-Resistant Staphylococcus aureus, United States 1999-2005. Emerging Infectious Diseases. Vol. 13(12): 1840-1846.

Lowy, F.D., 1998. Is Staphylococcus aureus an intracellular pathogen. Trends Microbiol 8: 341-344.

Malfanova, N., B. Lugtenberg and G. Berg. 2013. Bacterial Endophytes: Who and where, and what are they doing there?. To be published as a chapter in the book “Molecular Microbial Ecology of the Rhizosphereâ€, Wiley-Blackwell. P: 15-37.

Mayerhofer, M. 2011. Fungal Root Endophytes and Host Plant Growth. Heritage Brach. Canada. Pp : 17.

Radji, M. 2005. Peranan Bioteknologi dan Mikroba Endofit dalam Pengembangan Obat Herbal. Majalah Ilmu Kefarmasian. Vol. 2(3): 113-126.

Rasmann, S., Agrawal A.A. 2009. Plant defense against herbivory: progress in identifying synergism, redundancy, and antagonism between resistance traits. Current Opinion in Plant Biology 12: 473-478.

Rifai, M.A. 1992. Tiga puluh tumbuhan obat langka di Indonesia. Sisipan Florabunda 2. Penggalang taksonomi tumbuhan Indonesia, Bogor, h. 22-23.

Rosenblueth, M., and Martinez-Romero, E. 2006. Bacterial endophytes and their interactions with hosts. The American Phytophatological Society. 19(8): 827-837.

Sidik, S., E. Kurnia, dan Ursula. 1975. Usaha isolasi turunan kumarin dari akar purwoceng (Pimpinella alpina Molk.) asal dataran tinggi Dieng. Dalam Simposium Tanaman Obat I, 8-9 Desember, Bagian Farmakologi. FKH, Institut Pertanian Bogor.

Simarmata, R., Lekatompessy, S., dan Sukiman, H. 2007. Isolasi mikroba endofitik dari tanaman obat Sambung Nyawa (Gynura procumbens) dan analisis potensinya sebagai antimikroba. Berk. Penel.Hayati. 13: 85-90.

Strobel, G. dan Daisy, B. 2003. Bioprospecting for Microbial endophytes and their naturan product. Microbiology` and Molecular Reviews. 67(4) : 491-502.

Tan, R.X. dan W.X. Zou. 2001. Endophytes: a rich source of functional metabolites. Nat. Prod. Rep.18: 448–459.

Widayat, T., and Soetarto, A. E. S. 2012. Isolation of Endophyic Bacteria from Purwoceng (Pimpinella alpina Kds.). Health Science Indones. Vol 28(1): 31-36.

Wilson, W. 2014. Bakteri Endofit Tanaman Purwoceng (Pimpinella pruatjan Molk.) Berdasarkan Karakter Morfologis, Biokimiawi, dan Molekular. Tesis Program Studi Biologi, UGM. Yogyakarta.

Zinniel, D.K., Lambrecht, P., and Harris B.N., Feng, Z., Kuczmarski, D., Highley, P., Ishimaru, C.A., Arunakumari, A., Barletta, R.G., and Vidaver, A.K. 2002. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Applied and Environmental Microbiology. 68(5): 2198-2208.




DOI: https://doi.org/10.31327/jbse.v3i1.1465

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