Lentiviral shRNA Construction in Gene Therapy for Knock Down CYPIA1, and YWHAZ gene in Lung Cancer

Henik Anjayati(1*),

(1) Institut Teknologi Bandung
(*) Corresponding Author

Abstract


Lung cancer is a disease that is in the top 10 causes of death globally. It is caused by CYP1A1 and YWHAZ genes expressed in the lungs. One of the solutions offered for lung cancer sufferers is gene therapy. Gene therapy for lung cancer is focused on the knock-down of expressed CYP1A1 and YWHAZ genes. It can be using shRNA transfected into lung cells by lentivirus. The lentivirus envelope is modified using glycoprotein filovirus, one of which is ebolavirus. Lentivirus pseudotyping can recognize certain cells. Especially, Ebolavirus can recognize lung cells. The lentiviral gene is designed to carry the gene of interest (shRNA) but no the infectious gene. The shRNA gene transfused into lung cancer cells will recognize and complement the specific target mRNA sites of the CYP1A1 and YWHAZ genes. After that, shRNA will cut the mRNA which should be expressed into a cancer protein. Unexpressed mRNA into protein causes cancer cell growth inhibited. Thus, this gene therapy solution is expected to be able to reduce the rate of death caused by lung cancer.

 

Keywords: Lung cancer, CYP1A1 and YWHAZ genes, gene therapy, lentiviral, shRNA

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References


Abbas-Terki, T., Blanco-Bose, W., Déglon, N., Pralong, W., and Aebischer, P. (2002). Lentiviral-Mediated RNA Interference. Human Gene Therapy, 13: 2197–2201.

Addgene.org. https://www.addgene.org/guides/lentivirus/.

Boriss, H., and Kreith, M. (2007). Commodity Profile: Tobacco. Agricultural Issues Center University of California.

Brummelkamp, T.R., Bernards, R., and Agami, R. (2002). A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550–553.

Buchschacher, G.L., and Wong-Staal, F. (2018). Development of lentiviral vectors for gene therapy for human diseases. Blood, Vol. 95 (8).

Cancer.org. About Lung Cancer. American Cancer Sociaty. 1.800.227.2345. http://www.apastyle .org/products/index.aspx.

Cao, C., Chen, J., Lyu, C., Yu, J., Zhao, W., and Wang, Y. (2015). Bioinformatics Analysis of the Effects of Tobacco Smoke on Gene Expression. PLoSONE, 10(12): e0143377.doi:10.1371 /journal. pone.0143377.

Cellecta.com. https://www.cellecta.com/technologyportfolio/principles-of-rnai-and-shrna-design/.

Chan, S.Y., Speck, R.F., Ma, M.C., Goldsmith, M.A. (2000). Distinct mechanisms of entry by envelope glycoproteins of Marburg and Ebola (Zaire) viruses. J Virol, 74: 4933–4937. [PubMed: 10775638].

Chen, C., Chuang, S., Yang, M., Liao, J., Yu, S., and Chen, J.J.W. (2016). A Novel Function of YWHAZ/b-Catenin Axis in Promoting Epithelial–Mesenchymal Transition and Lung Cancer Metastasis. Angiogenesis, Metastasis, and the Cellular Microenvironment. DOI: 10.1158/1541-7786.MCR-12-0189.

Cronin, J., Zhang, x.y., and Reiser, j. (2005). Altering the Tropism of Lentiviral Vectors through Pseudotyping. Curr Gene Ther, Vol.5(4): 387–398.

Escors, D. and Breckpot, K. (2010). Lentiviral Vectors in Gene Therapy: Their Current Status and Future Potential. Arch. Immunol. Ther. Exp. 58:107–119 DOI 10.1007/s00005-010-0063-4.

Fan T, Li R, Todd NW, Qiu Q, Fang HB, Wang H, Shen J, Zhao RY, Caraway NP, Katz RL, Stass SA, Jiang F. Up-Regulation Of 14-3-3zeta in Lung Cancer and Its Implication as Prognostic and Therapeutic Target. Cancer Res, 67:7901–7906. [PubMed].

Genecards.org. https://www.genecards.org/cgi-bin/carddisp.pl?gene=CYP1A1#:~:text=CYP1 A1%20(Cytochrome%20P450%20Family%201,type%20and%20Arachidonic%20acid%20metabolism.

Hannonlab.cshl.edu. http://hannonlab. cshl.edu/rna/ shrna.pdf.

Hayafune, M., Miyano-Kurosaki, N., Park, W., Moori, Y., and Takaku, H. (2006). Silencing of HIV-1 Gene Expression by Two Types of siRNA Expression System. Antiviral Chemistry and Chemotherapy, 17: 241-249.

Hecht, Stephen S. (1999). Tobacco Smoke Carcinogens and Lung Cancer. Journal of the National Cancer Institute, Vol. 91 (14).

Jiang, X., Zhang, Q., Tian5, Y.H., Huang, J.C., And Ma, G.L. 2017. Rna Interference-Mediated Gene Silencing Of Cyclophilin A Enhances The Radiosensitivity of Paa Human Lung Adenocarcinoma Cells In Vitro. Oncology Letters. 13: 1619-1624. Doi: 10.3892/Ol.2017.5667.

Kouri, R. E., Demoise, C. F., and Whitmire, C., E. (1974). The Significance of Aryl Hydrocarbon Hydroxylase Enzyme Systems in the Selection of Model Systems for Respiratory Carcinogenesis. Experimental Lung Cancer.

Lu J, Guo H, Treekitk.rnmongkol W, Li P, Zhang J, Shi B, Ling C, Zhou X, Chen T, Chiao PJ, Feng X, Seewaldt VL, Muller WJ, Sahin A, Hung MC, Yu D. 14-3-3zeta Cooperates with Erbb2 to Promote Ductal Carcinoma in Situ Progression to Invasive Breast Cancer by Inducing Epithelial-Mesenchymal Transition. Cancer Cell, 16:195–207. [PMC free article] [PubMed].

Malacards.org. https://www.malacards.org/card/aryl_hydrocarbon_hydroxylase_inducibility.

Martin, S.E., Jones, T.L., Thomas, C.L., Lorenzi, P.L., Nguyen, D.A., Runfola, T., Gunsior, M., Weinstein, J.N., Goldsmith, P.K., Lader, E., Huppi, K., and Caplen, N.J. (2007). Multiplexing siRNAs to compress RNAi-based screen size in human cells. Nucleic Acids Res. 35: e57.

Matta A, DeSouza LV, Shukla NK, Gupta SD, Ralhan R, Siu KW (2008) Prognostic Significance of Head-And-Neck Cancer Biomarkers Previously Discovered and Identified Using Itraq-Labeling and Multidimensional Liquid Chromatography-Tandem Mass Spectrometry. J Proteome Res, 7(5): 2078–2087.

McIntyre, G.J. and Fanning, G.C. (2006). Design and cloning strategies for constructing shRNA expression vectors. MC Biotechnology. 6:1. https://doi.org/10.1186/1472-6750-6-1.

Merten, O., Hebben, M., and Bovolenta, C. (2016)., Production of lentiviral vectors. Mol Ther Methods Clin Dev. 2016; 3: 16017. doi: [10.1038/mtm.2016.17].

Milone1, M.C. and O’Doherty, U. (2018). Clinical use of lentiviral vectors. Leukemia, 32: 1529–1541 https://doi.org/10.1038/s41375-018-0106-0.

Mohammed, K. and Shervingto. A. (2008). Can CYP1A1 shRNA Be an Effective Treatment for Lung Cancer? Cellular & Molecular Biology Letters. Vol.13: pp 240-249. DOI: 10.2478/s11658-007-0050-x.

Moore, C.B., Guthrie, E.H., Huang, M. and Debra J. Tn. (2010). Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown. Methods Mol Biol. 629: 141–158. doi:10.1007/978-1-60761-657-3_10.

Morris, K.V., and Rossi, J.J. (2006). Lentiviral-mediated delivery of siRNAs for antiviral therapy. Gene Therapy, 13: 553–558.

Najm, M. Z., Zaidi, S., Akhtar, M. S., and Siddiqui, W. (2013). Mutation and protein expression analysis of CYP1A1 gene—a study on female breast cancer cases from India. Tumor Biol. DOI 10.1007/s13277-013-1262-5.

Neal, C.L., Yao, J., Yang, W., Zhou, X., Nguyen, N.T., Lu, J., Danes, C.G., Guo, H., Lan, K. H,, Ensor, J., Hittelman, W., Hung, M.C., and Yu, D. (2009). 14-3-3zeta. Overexpression Defines High Risk for Breast Cancer Recurrence and Promotes Cancer Cell Survival. Cancer Res, 69(8): 3425–3432.

Neal, R.D. (2019). Lung Cancer. BMJ, 365. doi: 10.1136/bmj.l1725.

O’Keefe, E. P. (2013). siRNAs and shRNAs: Tools for Protein Knockdown by Gene Silencing. DOI. //dx.doi.org/10.13070/mm.en.3.197.

Origene.com. https://www.origene.com/products/vectors/ shrna-vectors.

Patil, P.M., Chaudhari P. D., Sahu M., and Duragkar N. J. (2012). Review Article on Gene Therapy. International Journal of Genetics, Vol 4 (1). 74-79.

Paddison, P., Caudy, A.A., Bernstein, E., Hannon, G.J., and Conklin, D.S. (2002). Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 2002 Apr 15; 16(8): 948–958. doi: [10.1101/gad.981002].

Prabandari, Y.S., and Dewi, A. (2016). How do Indonesian Youth Perceive Cigarette Advertising? A Cross-Sectional Study Among Indonesian High School Students. Glob Health Action, 9: 30914. http://dx.doi.org/10.3402 /gha.v9.30914.

Rachmat, M. (2010). Development of National Tobacco Economy: Developed Country Policy and Lesson Learned for Indonesia. Analisis Kebijakan Pertanian, Vol. 8 (1). 67-83.

Santes-Palacios, R., Ornelas-Ayala, D., Cabañas, N., Marroquín-Pérez, A., Hernández-Magaña, A., Olguín-Reyes, S.R., Camacho-Carranza, R., and Espinosa-Aguirre, J.J. (2016). Regulation of Human Cytochrome P4501A1 (hCYP1A1): A Plausible Target for Chemoprevention? BioMed Research International, Volume 2016, Article ID 5341081, 17 pages http://dx.doi.org/10.1155/2016 /5341081.

Spanevello, F., Calistri, A., Vecchio, C.D., Mantelli, B., Frasson, C., Basso, G., Palù, G., Cavazzana, M., and Parolin, C. 2016. Development of Lentiviral Vectors Simultaneously Expressing Multiple siRNAs Against CCR5, vif and tat/rev Genes for an HIV-1 Gene Therapy Approach. Molecular Therapy-Nucleic Acids. 5, e312; doi: 10.1038/mtna.2016.24.

Sui, G., Soohoo, C., Affar El, B., Gay, F., Shi, Y., and Forrester, W.C. (2002). A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 99, 5515–5520.

Wang, D., Wang, L., Zhou, J., Pan, J., and Qian, W. (2014) Reduced Expression of PTPRD Correlates with Poor Prognosis in Gastric Adenocarcinoma. PLoS ONE, 9(11): e113754. doi:10.1371/ journal.pone.0113754.

Watanabe, N., Komatsu, S., Miyamae, M., Ohashi, T., Okajima, W., Kosuga, T., Konishi, H., Shiozaki, A., Fujiwara, H., Okamoto, K.,Tsuda, H., and Otsuji, E. (2016). Overexpression of YWHAZ as an Independent Prognostic Factor in Adenocarcinoma of the Esophago-Gastric Junction. Am J Cancer Res., 6(11): 2729–2736. PMCID: PMC5126287. PMID: 27904785.

Yu, J.Y., Deruiter, S.L., and Turner, D.L. (2002). RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 99, 6047–6052.




DOI: https://doi.org/10.31327/jbse.v3i1.1436

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