Spermatozoa Quality of Kampung Unggul Balitbangtan (KUB) Chicken at 5oC Storage Supplemented with Glucose in Liquid Semen Diluent

J. Junaedi(1*), Nurul Isnaini(2), Muhammad Halim Natsir(3), I. Iswati(4), Trinil Susilawati(5),

(1) Universitas Sembilanbelas November Kolaka
(2) Universitas Brawijaya
(3) Universitas Brawijaya
(4) Politeknik Pembangunan Pertanian Malang
(5) Universitas Brawijaya
(*) Corresponding Author

Abstract


This research was conducted to determine the effect of glucose supplementation in liquid semen diluent on the quality of Kampung Unggul Balitbangtan (KUB) spermatozoa.The chicken used is KUB chicken as many as 8 tails.The research procedure consists of; the manufacture of diluent, collection of semen, dilution of semen, storage, and evaluation of semen.Evaluation of semen was carried out on motility, viability, abnormalities, and plasma membrane integrity at 0 and 3 days of storage.Data were analyzed using the T-test to compare the quality of spermatozoa with added glucose into the liquid semen diluent and without the addition of glucose.The addition of glucose into the diluent was able to maintain spermatozoa motility at 35.00 ± 11.50, spermatozoa viability at 41.42 ± 10.15, and spermatozoa plasma membrane integrity at 82.94 ± 7.42 at 5oC storage for three days.This study concluded that glucose supplementation into liquid semen diluent was able to maintain plasma membrane motility, viability, and integrity at 5oC storage for three days, spermatozoa quality of KUB chickens was able to survive at 5oC storage for three days, and glucose can be added to the liquid semen diluent at a dose of 10 mM.


Keywords


Kampung Unggul Balitbangtan, chicken, glucose, liquid semen

Full Text:

PDF

References


Cerolini, S., Zainiboni, L., Maldjian, A., & Gliozzi, T. (2006). Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology, 66, pp. 877–886.

Etches, R. J. (1996). Reproduction in Poultry. Cambridge, UK: CABI, University Press.

Getachew, T. (2016). A review article on artificial insemination in poultry. World Vet J, 6(1), 25–33.

Garner, D. L. & Hafez, E. S. E. (2000). Spermatozoa and Seminal Plasma. In: Reproduction in Farm Animals. 7th ed. E. S. E. Hafez (ed.). Lea & Febiger, Philadelphia, pp. 96-125.

Junaedi, J., Arifiantini, R. I., Sumantri, C. & Gunawan, A. (2016a). Use of Glycerol as Cryoprotectants in Freezing Sentul Chicken Semen. Chalaza Journal of Animal Husbandry, 1(2), pp. 6-13. DOI: https://doi.org/10.31327/chalaza.v1i2.165

Junaedi, J., Arifiantini, R. I., Sumantri, C., & Gunawan, A. (2016b). Penggunaan Dimethyl Sulfoxide Sebagai Krioprotektan dalam Pembekuan Semen Ayam Kampung. Jurnal Veteriner, 17(2), pp. 300-308.

Lake, P. E., & Wishart, G. J. (1984). Comparative physiology of turkey and fowl semen. In F. J. Cunningham, P. E. Lake, & D. Hewit (Eds.), Reproductive Biology of Poultry. Harlow, UK: British Poultry Science, pp. 151–160.

Long, J. A., & Kramer, M. (2003). Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry Science, 82, pp. 1802–1807.

Manjunath, P. (2012). New insight into the understanding of the mechanism of sperm protection by extender components. Anim Reprod, 9(4), pp. 809–815.

Moussa, M., Martinet, V., Trimeche, A., Tainturier, D., & Anton, M. (2002). Low-density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective. Theriogenology, 57, pp. 1695–1706.

Mohan, J., Sharma, S. K., Kolluri, G., & Dhama, K. (2018). History of artificial insemination in poultry, its components, and significance. World’s Poultry Science Journal, 74, pp. 475–488.

Santiago-Moreno, J., Castaño, C., Toledano-Díaz, A., Coloma, M. A., López-Sebastián, A., Prieto, M. T., & Campo, J. L. (2012). Cryoprotective and contraceptive properties of egg yolk as an additive in rooster sperm diluents. Cryobiology, 65(3), pp. 230–234.

Setiawan, R., Priyadarshana, C., Tajima, A., Travis, A. J., & Asano, A. (2020). Localization and function of glucose transporter GLUT1 in chicken (Gallus gallus domesticus) spermatozoa: the relationship between ATP production pathways and flagellar motility. Reproduction, Fertility and Development, 32(7), pp. 697–705.

Soler A. J., Guzman, M. D. P., & Garde, J. J. (2003). Storage of red-deer epididymides for four days at 5oC: effects on sperm motility, viability, and morphology integrity. J. Exp. Zool. 295A, pp. 188-199.

Sudjana. (2005). Metode Statistika. Bandung, Indonesia: Tarsito.

Toelihere, M.R. (1993). Fisiologi Reproduksi pada Ternak. Bandung, Indonesia: Penerbit Angkasa.

Toelihere, M R. (1985). Inseminasi Buatan pada Ternak. Bandung, Indonesia: Penerbit Angkasa.

Ushiyama, A., Priyadarshana, C., Setiawan, R., Miyazaki, H., Ishikawa, N., Tajima, A., & Asano, A. (2019). Membrane raft-mediated regulation of glucose signaling pathway leading to acrosome reaction in chicken sperm. Biol. Reprod., 100, pp. 1482–1491.




DOI: https://doi.org/10.31327/chalaza.v8i1.1932

Article Metrics

Abstract view : 105 times
PDF - 58 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Chalaza Journal of Animal Husbandry All rights reserved

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

     

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Chlaza Journal of Animal Husbandry, Jl. Pemuda, No. 339 Kolaka, Southeast Sulawesi, Indonesia - 93517 Mobile: +6285255092481 WA: +6285255092481 E-mail : editor.cjah@usnsj.com or chalaza.usnsj@gmail.com

ONLINE ISSN: 2548-3803 PRINT ISSN: 2548-5504


Universitas Sembilanbelas November Kolaka, USN Scientific Journal Publisher (Hardcopy Provider)
 Jl. Pemuda, No. 339 Kolaka, Southeast Sulawesi, Indonesia - 93517 E-mail: info@usnsj.com

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Chalaza Journal of Animal Husbandry licensed under a Creative Commons Attribution 4.0 International License.

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------