Effect of Caffeine on Motility of Epididymis Spermatozoa of Bali Bull in Slaughterhouse Cibinong

Oktora Dwi Putranti(1*), Lovita Adriani(2), S. Soeparna(3), Tita Damayanti Lestari(4),

(1) Universitas Khairun Ternate
(2) Universitas Padjadjaran, Bandung
(3) Universitas Padjadjaran, Bandung
(4) Universitas Padjadjaran, Bandung
(*) Corresponding Author

Abstract


Abattoir is the place to get meat but also a source of potential genetic sperm. Sperm from a slaughterhouse has low motility. Sperm motility can be improved by adding caffeine to the thinner before being used for fertilization. Caffeine is an alkaloid compound that can increase energy through a cAMP cycle. The method used is the testis of 12 cows Bali taken from a slaughterhouse Cibinong and do frozen sperm. Frozen sperm is analyzed using a computer-assisted sperm Analyzed (CASA) who had been treated caffeine 0, 2, 4, and 6 mg/ml. Fertility frozen epididymis sperm was tested using in vitro fertilization. Results were analyzed using a completely randomized design (CRD) with four treatments unidirectional pattern three repetitions. The results showed that the treatment with the addition of caffeine to the thinner of the yolk tris egg yolk epididymis sperm, there was no difference in motility, recovery rate, Curvilinear velocity (VCL), average path velocity (VAP), and straight-line velocity (VSL).

Keywords


cauda epididymis sperm, motility, caffeine

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References


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DOI: https://doi.org/10.31327/chalaza.v4i2.1133

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Chlaza Journal of Animal Husbandry, Jl. Pemuda, No. 339 Kolaka, Southeast Sulawesi, Indonesia - 93517 Mobile: +6285255092481 WA: +6285255092481 E-mail : editor.cjah@usnsj.com or chalaza.usnsj@gmail.com

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Chalaza Journal of Animal Husbandry licensed under a Creative Commons Attribution 4.0 International License.

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