Effect of Caffeine on Motility of Epididymis Spermatozoa of Bali Bull in Slaughterhouse Cibinong

Oktora Dwi Putranti(1*), Lovita Adriani(2), S. Soeparna(3), Tita Damayanti Lestari(4),

(1) Universitas Khairun Ternate
(2) Universitas Padjadjaran, Bandung
(3) Universitas Padjadjaran, Bandung
(4) Universitas Padjadjaran, Bandung
(*) Corresponding Author

Abstract


Abattoir is the place to get meat but also a source of potential genetic sperm. Sperm from a slaughterhouse has low motility. Sperm motility can be improved by adding caffeine to the thinner before being used for fertilization. Caffeine is an alkaloid compound that can increase energy through a cAMP cycle. The method used is the testis of 12 cows Bali taken from a slaughterhouse Cibinong and do frozen sperm. Frozen sperm is analyzed using a computer-assisted sperm Analyzed (CASA) who had been treated caffeine 0, 2, 4, and 6 mg/ml. Fertility frozen epididymis sperm was tested using in vitro fertilization. Results were analyzed using a completely randomized design (CRD) with four treatments unidirectional pattern three repetitions. The results showed that the treatment with the addition of caffeine to the thinner of the yolk tris egg yolk epididymis sperm, there was no difference in motility, recovery rate, Curvilinear velocity (VCL), average path velocity (VAP), and straight-line velocity (VSL).

Keywords


cauda epididymis sperm, motility, caffeine

Full Text:

PDF

References


Arifiantini, I. (2012). Teknik Koleksi dan Evaluasi Semen Pada Hewan. Bogor, Indonesia: Institut Pertanian Bogor Press.

Garner, D. L, & Hafez, E. S. E. (2000). Sperm and Seminal Plasma: in Hafez, B, and Hafez, E.S.E: Reproduction in Farm Animals. New York, USA: Lippincott Williams & Wilkins, pp. 97-105.

Hasbi, Sonjaya, H., & Gustina, S. (2011). Pengaruh Medium Pemisah, Penambahan Ekstrak Kopi Sebelum Proses Pemisahan Sperma Pembawa Kromosom X dan Y Dan Lama Penyimpanan Terhadap Kualitas Semen cair Kambing Peranakan Ettawa. Makassar, Indonesia: Fakultas Peternakan Universitas Hasanuddin.

Putranti, O. D. (2016). Pengaruh Penambahan Kafein Pada Spermatozoa Kauda Epididimis Sapi Bali Pasca Thawing Terhadap Fertilitas Secara Fertilisasi In Vitro. Disertasion. Bandung, Indonesia: Universitas Padjadjaran.

Sarastina, T. Susilawati, G. Ciptadi. 2014. Analisa Beberapa Parameter Motilitas Sperma Pada Beberapa Bangsa Sapi Menggunakan Computer Assisted Semen Analysis (CASA). Jurnal Ternak Tropika. Vol. 6 (2), pp. 1-12.

Sukmawati, E. Arifiantini, R. I., & Purwantara, B. (2014). Daya tahan sperma terhadap proses pembekuan pada berbagai jenis sapi pejantan unggul. Jurnal Ilmu Ternak Veteriner. Vol. 19 (3), pp. 168-175.

Trihendradi, C. (2010). Step by Step Spss 18 Analisis Data Statistik. Yogyakarta, Indonesia: Andy Offset.




DOI: https://doi.org/10.31327/chalaza.v4i2.1133

Article Metrics

Abstract view : 426 times
PDF - 199 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 CJAH All rights reserved

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

     

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Chlaza Journal of Animal Husbandry, Jl. Pemuda, No. 339 Kolaka, Southeast Sulawesi, Indonesia - 93517 Mobile: +6285255092481 WA: +6285255092481 E-mail : editor.cjah@usnsj.com or chalaza.usnsj@gmail.com

ONLINE ISSN: 2548-3803 PRINT ISSN: 2548-5504


Universitas Sembilanbelas November Kolaka, USN Scientific Journal Publisher (Hardcopy Provider)
 Jl. Pemuda, No. 339 Kolaka, Southeast Sulawesi, Indonesia - 93517 E-mail: info@usnsj.com

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Chalaza Journal of Animal Husbandry licensed under a Creative Commons Attribution 4.0 International License.

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------